Lippia sidoides Cham. is widely used in North-East Brazil to treat oral and vaginal infectious diseases due to its strong antimicrobial activity. The aim of this study. Chemical Constituents of Essential Oil from Lippia sidoides Cham. (Verbenaceae ) Leaves Cultivated in Hidrolândia, Goiás, Brazil. Braz J Med Biol Res, March , Volume 40(3) Antimicrobial activity of the essential oil from Lippia sidoides, carvacrol and thymol against oral.

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Antimicrobial activity sidoidea Lippia sidoides Cham. Verbenaceae essential oil against Staphylococcus aureus and Escherichia coli. Verbenaceae contra Staphylococcus aureus e Escherichia lippua. The antibacterial effect of Lippia sidoides rosemary pepper essential oil was tested against the bacteria Staphylococcus aureus sidoidess Escherichia coli isolated from homemade Minas cheese produced in Brazil.

Results sioides the bacterial activity of Lippia sidoides essential oil against S. The industry has tried to reduce the use sidoidex chemical preservatives due to the increasing legal demands from law-enforcement agencies and the health risk for consumers.

Several plants have been evaluated and presented promising results as preservatives and preventers of pathogenic microorganism growth in food, including Salmonella enteritidis and Staphylococcus aureus Tassou et al.

The utilization of natural antibacterial products is a promising alternative for the control of pathogenic bacteria in food, reducing sanitary risks and economic losses, besides prolonging shelf life. The antimicrobial activity of essential oils from medicinal plants is performed by a group of terpenoids and phenols. This property is associated with their lipolytic characteristic, causing accumulation on the cell membrane and injuries associated with energy depletion Oussalah et al.

Antimicrobial activity may be related to the composition and structural configuration of the plant volatile oils, their functional groups and the possibility of synergism among their compounds. Great differences among the results for oils obtained from the same plant have already been reported.

This variation is associated with geographical variability, genotype, climate and laboratory methods utilized to extract the oil. These factors may interfere in the chemical composition and proportion of individual compounds of the plant essential oil Oussalah et al. Essential oils from plants have antimicrobial activity aidoides a large number of bacteria, including strains resistant to antibiotics and antifungals Bertini et al. In addition, the oil content may vary according to the harvest time, location, temperature and other environmental factors.

This study aimed to evaluate the antimicrobial activity and antiseptic potential of Lippia sidoides Cham.

Thirty Staphylococcus aureus and thirty Escherichia coli isolates, obtained from 72 samples of homemade Minas cheese, were studied. The hydrodistillation technique with Clevenger-type apparatus was used to obtain Lippia sidoides Cham.


The extracted oil was stored in sterile dark flasks to maintain its phytochemical properties. After the growth of microorganisms, inocula were prepared in 0. The tests were conducted in triplicate. The results were compared with three controls: Sidoixes Minimum Inhibitory Concentration MICwhich is the smallest concentration of essential oil capable of inhibiting the growth of the tested microorganisms, was assessed according to Oussalah et al.

The Minimum Bactericidal Concentration MBC was determined according to the agar plating technique for counting all the oil dilutions that did not present growth for MIC. Results were expressed as Xidoides mL The disk diffusion technique Sidoices et al.

The diffusion test was conducted by scattering on Mueller Hinton plate count agar; inocula were standardized to 0. The samples were compared to the negative and positive controls and the solvent. According to this author, to assure a satisfactory antimicrobial activity, sidiodes product should present a microbicidal effect ME higher than or equal to 5 in suspension tests after a contact period of five minutes.

ME was calculated by using the luppia formula: An aliquot was taken from this mixture and added to 0. Then, the cultures were diluted in 0. After the incubation period, the number of CFU was determined.

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The essential of Lippia sidoides presented a bactericidal effect against S. Several studies have determined the MIC of essential oils in order to establish the balance between acceptability and antimicrobial efficiency Nascimento et al.

Using the microdilution technique, Oliveira et al. The chemical composition of essential oils may be influenced by climate, season, geographical conditions, harvest time and distillation technique. Studies done by Bertini et al. Sixoides this study, however, the obtained MIC was the same for both gram-positive and gram-negative bacteria, proving thus the efficacy of Lippia sidoides essential oil against both bacterial agents.

International Journal of Analytical Chemistry

The results sidoives the inhibition halo size in the test of microbial sensitivity in disk are presented in Table 3. According to MIC determination in the disk, the size of inhibition halos differed between E. The disks impregnated with crude oil formed inhibition halos of 23 mm and 26 mm for E. Gram-negative bacteria have a less rigid and chemically less complex cell wall than gram-positive ones, besides presenting higher lipid content Vargas et al.

This may be related to the stronger resistance eidoides E. The microbicidal action of Lippia sidoides essential oil was efficient against all tested bacteria. There was no significant difference in the microbicidal effect of Lippia sidoides oil on the tested bacteria.


Lippia – Wikipedia

These results suggest that Lippia sidoides oil is efficient against the bacteria Staphylococcus aureus and Escherichia coli isolated from homemade cheese. In food, essential oils may migrate to the lipid segment, reducing thus their concentration lipoia the aqueous phase and consequently their contact with microorganisms located in the hydrophilic parts of the food.

These interferences require higher concentrations of essential oil for a good antiseptic activity in food. The use of Lippia sidoides essential oil as a component in sanitizing products for food industry may be an efficient and economical alternative for the cleaning of equipment and utensil surfaces. An alternative to reduce the loss of Lippia sidoides sanitary sidoldes during processing, storage and commercialization would be the use of encapsulation technologies combined with bioactive and biodegradable packing, so that the active components could be released during consumption.

In these packs, bioactive components are fixed in a biodegradable matrix which releases the components through the food humidity Castro et al.

The results of this study showed that Lippia sidoides essential oil presents good antiseptic properties against the lippoa microorganisms. In addition, other experiments may be conducted to test the effect of lower concentrations in longer contact with microbial cells. Studies may also be proposed to determine better strategies to make the use of Sidiides sidoides essential oil as antiseptic in the food industry more technologically and economically viable.

Evaluation of the repeatability and reproducibility of European suspension test methods for antimicrobial activity of disinfectants and antiseptics. The Journal of Applied Bacteriology sidodes, v. Antimicrobial agents from plants: Journal of Applied Microbiologyv.

Revista Brasileira de Farmacognosiav. Effectiveness of Lippia sidoides Cham. Verbenaceae essential oil in inhibiting the growth of Staphylococcus aureus strains isolated from clinical material. Brazilian Journal of Pharmacognosyv. Antimicrobial effects of selected plant essential oils on the growth of a Pseudomonas putida strain isolated from meat. Meat Sciencev. Inhibition of Salmonella enteritidis and Staphylococcus aureus in nutrient broth by mint essential oil.

Food Research Internationalv. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. Services on Demand Journal. How to cite this article.